Super-resolution imaging via sparsity constraint and sparse speckle illumination
Wang Pengwei1, 2, Li Wei2, 3, Wang Chenglong1, 2, Bo Zunwang1, Gong Wenlin1, †
Key Laboratory for Quantum Optics and Center for Cold Atom Physics, Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai 201800, China
University of Chinese Academy of Sciences, Beijing 100049, China
Academy of Opto-electronics, Chinese Academy of Sciences, Beijing 100094, China

 

† Corresponding author. E-mail: gongwl@siom.ac.cn

Project supported by the National Natural Science Foundation of China (Grant No. 61571427).

Abstract

We present an imaging approach via sparsity constraint and sparse speckle illumination which can dramatically enhance the optical system’s imaging resolution. When the object is illuminated by some sparse speckles and the sparse reconstruction algorithm is utilized to restore the blur image, numerical simulated results demonstrate that the image, whose resolution exceeds the Rayleigh limit, can be stably reconstructed even if the detection signal-to-noise ratio (SNR) is less than 10 dB. Factors affecting the quality of the reconstructed image, such as the coded pattern’s sparsity and the detection SNR, are also studied.

PACS: 42.30.-d
1. Introduction

Images with high spatial resolution are always required in most imaging applications especially in life science, material science, and remote sensing. For conventional imaging, the optical system’s imaging resolution is restricted by the imaging system’s Rayleigh limit and the camera’s pixel resolution.[1] A series of methods are proposed to break the optical system’s Rayleigh limit, which is often called super-resolution imaging. Generally speaking, super-resolution reconstruction approaches can be divided into two cases. One is single-frame super-resolution reconstruction, and the other is multi-frame super-resolution reconstruction.[2] For single-frame super-resolution reconstruction, the light intensity distribution illuminating the object is usually uniform, and some prior information like sparsity and probability density is utilized.[27] For multi-frame super-resolution reconstruction, the light intensity distribution on the object for each frame may be uniform or heterogeneous. For the former, the imaging resolution is enhanced because the object’s high-frequency can be obtained by looking at the same object from different angles.[8] For the latter, super-resolution imaging can be obtained based on the random and sparsity property of the speckle illuminating the object. Examples include the ghost imaging,[9,10] fluorescence imaging,[11,12] and structured illumination microscopy.[1315] However, the improvement of imaging resolution for the super-resolution reconstruction approaches described above is limited in practice because of the effect of detection noise or the complexity of the object.[1618] Therefore, it is natural to ask whether some robust super-resolution imaging methods can be developed by adopting the mutual advantages of present super-resolution reconstruction techniques. For example, the sparse reconstruction algorithm has a great advantage to obtain super-resolution images of sparse objects.[35] If the object is not sparse, we can use some sparse speckle patterns to illuminate the object, then each image transmitted/reflected from the object becomes sparse and super-resolution reconstruction of complex objects can also be realized by the sparse reconstruction algorithm. Also, high-order correlated imaging can improve the system’s imaging resolution to some extent,[19] which is possible to further improve the imaging resolution of super-resolution imaging via sparse speckle illumination.

In this paper, by combining the advantage of sparse speckle illumination with the characteristic of high-order correlated imaging, a super-resolution imaging approach via sparsity constraint and sparse speckle illumination is proposed. We have demonstrated by numerical simulation the super-resolution ability of the proposed approach. The influences of the coded pattern’s sparsity at the object plane and the detection signal-to-noise ratio (SNR) to the reconstruction quality are also discussed.

2. Model of the proposed approach

A typical optical imaging system, as shown in Fig. 1(a), usually consists of an illumination source, an object, an imaging component, and a detector. When the illumination light is fully spatially incoherent, the intensity at the detection plane is the convolution of the intensity transmitted from the object with the incoherent point spread function (PSF)[16]

where ⊗ denotes the convolution operation, S(x,y) is the intensity distribution of the light field illuminating the object, h(x,y) is the optical imaging system’s PSF, n(x,y) = 10β max{[S(x,y) · Iobj(x,y)] ⊗ h(x,y)}G(x,y) (the entries of G(x,y) satisfy a Gaussian distribution with mean 0 and variance 1) denotes the detection noise, and the detection SNR is 10β. In addition, Iobj(x,y) is the object’s transmission function, and Iim(x,y) is the image intensity.

Fig. 1. (color online) (a) The schematic of a typical optical imaging system. (b) Single-frame super-resolution reconstruction via uniform illumination. (c) Procedure of super-resolution imaging via sparsity constraint and spare speckle illumination. CCD: charge-coupled device; PI: projection illumination; DI: diffraction-limited; SR: sparse reconstruction; S1SN are the random sparse speckle patterns; and Σ denotes the summation.

For the schematic of single-frame super-resolution reconstruction shown in Fig. 1(b), S(x,y) is uniform and we have implemented the Lucy–Richardson algorithm in the image reconstruction process.[20,21] The reconstruction algorithm is based on the Bayesian analysis and the assumption that each image’s pixel follows the Poisson distribution. Then the original target is reconstructed by maximizing the posterior probability according to the blur image and the system’s PSF. The optimization process can be expressed as

where Irec(x,y) is the reconstruction result.

For the proposed method of super-resolution imaging via sparse speckle illumination depicted in Fig. 1(c), a series of random sparse speckle patterns S1(x,y)–SN(x,y) illuminate the same object in turn. Because the optical system is diffraction-limited, we can obtain a sequence of blur images correspondingly, namely,

where Si(x,y) and are the intensity distribution of the sparse speckle pattern illuminating the object and the image transmitted from the object in the i-th frame, respectively. In addition, N is the total frame number and S(x,y) is the total intensity distribution of the sparse speckle patterns at the object plane. In order to guarantee the gray fidelity of the reconstructed image, S(x,y) is usually set to be homogeneous and there is no speckles’ overlap between any two frames. Therefore, the speckle area a × a, the total frame number N, the speckle’s number K (the number of bright spots per frame), and the reconstruction area L × L satisfy the relation L × L = N × K × a × a.

According to each blur image , we can obtain corresponding by the reconstruction algorithm of Eq. (2). For multi-frame imaging via speckle illumination, high-order correlated imaging can improve the system’s imaging resolution to some extent compared with the traditional directly integral imaging,[19] hence the final image is also reconstructed by calculating the high-order correlation function of the light field, which is called as super-resolution imaging via sparsity constraint and sparse speckle illumination (SISC-SSI), namely,

where represents the ensemble average of .

In summary, the reconstruction procedure of SISC-SSI can be expressed as the following three steps:

Obtain a sequence of blur images by Eq. (3).

Reconstruct by Eq. (2) according to and PSF.

Obtain the final image ISISC−SSI(x,y) by Eq. (5).

3. Simulation results and discussion

Figure 2 presents the principle schematic of SISC-SSI. The uniform and incoherent light emitted from a xenon lamp is collimated by a lens with a focal length of f0 and then filtered by an optical filter (with a center wavelength of λ = 532 nm). The light transmitted from the filter propagates to a digital micro-mirror device (DMD). A series of coded patterns, as shown in Fig. 1(c), are pre-built by modulating the DMD and then imaged onto the object by the lens with the focal length f1 = 75 mm. The light transmitted from the object is imaged onto a charge-coupled device (CCD) camera by a 4f optical system. An Iris with the diameter D is placed at the focal plane of the lens f = 250 mm, which is used to control the system’s diffraction limit. In the simulation experiment, the resolution test chart (Fig. 3(a)) is utilized as the object, the object is composed of four sets of different slit widths (120 × 120 pixels, the pixel size is 3.45 μm × 3.45 μm). The object’s center-to-center separation is d1:d2:d3:d4 = 1:2:3:4 and d1 = 13.8 μm. The image recorded by the CCD camera has 240 × 240 pixels and the pixel size of the CCD camera is also 3.45 μm × 3.45 μm. In addition, the coded region on the DMD is 60 × 60 pixels and the pixel size of the DMD is 13.68 μm × 13.68 μm. In order to match the speckle’s transverse size at the object plane with the pixel size of the CCD camera, the distances z1 and z2 are set as 223.7 mm and 112.8 mm, respectively, which means that the speckle’s transverse size illuminating on the object is 6.9 μm.

Fig. 2. (color online) Schematic of super-resolution imaging via sparsity constraint and sparse speckle illumination.
Fig. 3. (color online) (a1) Original object. (a2) The PSF corresponding to a spatial resolution of 41.4 μm. (a3) Cross section of PSF along (x,y = 0) of (a2). (b1)–(b5) The images recorded by the CCD camera in different detection SNR (the image area labeled by the red rectangular box is 120 × 120 pixels). (c1)–(c5) Single-frame reconstruction results via uniform illumination (the detection area recorded by the CCD camera is 240 × 240 pixels). (d1)–(d5) Single-frame reconstruction results via uniform illumination based on the detection data shown in the red rectangular box. (e1)–(e5) One frame of detection image in different detection SNR for SISC-SSI. (f1)–(f5) The reconstruction results corresponding to the images of (e1)–(e5). (g1)–(g5) The reconstruction results of SISC-SSI in different detection SNR when the speckle’s number is 5 for each frame. From left to right, the detection SNR is ∞, 20 dB, 15 dB, 10 dB, and 5 dB, respectively.

Under a uniform illumination, we can adjust the Iris’s transmission aperture to make some details of the target undistinguished (the spatial resolution is about d3 = 41.4 μm). In this case, the transverse size of the Iris is approximately D ≈ 1.22λ f/d3 = 3.9 mm and the optical system’s PSF is displayed in Figs. 3(a2) and 3(a3). Correspondingly, the blur image of the object in different detection SNR is illustrated in Figs. 3(b1)3(b5). Based on Eq. (2), when the detection area on the CCD camera is chosen as 240 × 240 pixels and 120 × 120 pixels, figures 3(c1)3(c5) and 3(d1)3(d5) give the corresponding single-frame reconstruction results, respectively. It is clearly seen that the object’s image can be restored without noise when the detection area is twice as large as the object area (Fig. 3(c1)), whereas single-frame reconstruction is disabled even if the detection SNR reaches 20 dB (Fig. 3(c2)). In contrast with Figs. 3(c1)3(c5), when the detection area is the same as the object area (the area labeled by the red rectangular box in Figs. 3(b1)3(b5), the object can not be restored even without noise (Figs. 3(d1)3(d5)). Correspondingly, when the object is illuminated by some random sparse speckle and the detection area is the same as the object area, figures 3(e1)3(e5) present one frame of detection image in different detection SNR and the corresponding recovered results based on Eq. (2) are shown in Figs. 3(f1)3(f5). When the speckle number is 5 in each frame, the reconstruction results of SISC-SSI are displayed in Figs. 3(g1)3(g5). It is obvious that SISC-SSI can stably reconstruct the object and robust to noise. Therefore, we have demonstrated the validity of our super-resolution approach and the imaging resolution can be smaller than the diffraction limit by 1/3.

In Fig. 4, we show the simulated SISC-SSI reconstruction results of imaging the same object displayed in Fig. 3(a) when the coded pattern’s sparsity (the speckle’s number at the object plane) is different and the detection SNR is 10 dB. In Fig. 4(a), each illumination has only one speckle, which is the same as point scanning imaging[22] and has the best reconstruction quality because each image restored is the sparsest.[3,4,9] However, the measurement number N is the largest. From Figs. 4(a)4(f), we can find that as the speckle’s number illuminating the object is increased, the reconstruction resolution will decay.

Fig. 4. (color online) The influence of the speckle’s number illuminating on the object to SISC-SSI when the detection SNR is 10 dB for each frame. The speckle’s number is 1, 5, 15, 30, 60, and 120 for (a)–(f), which is corresponding to N = 3600, 720, 240, 120, 60, and 30, respectively.

In order to evaluate quantitatively the influence of the speckle’s number and detection SNR to the reconstructed results of SISC-SSI, the reconstruction quality is estimated by calculating the peak signal-to-noise ratio (PSNR)

where the bigger the PSNR is, the better the quality of the recovered image is. For a 0–255 gray-scale image, p = 8 and MSE represents the mean square error of the reconstruction image ISISC–SSI with respect to the original object Iobj
Here Npix is the total pixel number of the image. From Fig. 5, it is obviously seen that the PSNR always increases with the detection SNR and decreases with the speckle’s number at the object plane, which is also consistent with the results indicated in Figs. 3 and 4.

Fig. 5. (color online) The relationship between PSNR and the speckle’s number in different detection SNR.

To validate the applicability of SISC-SSI for more general images, we give a numerical experimental demonstration of imaging a continuous varying gray-scale object, i.e., a slide representing a detail of the picture “lena” (120 × 120 pixels). Figures 6(b1)6(b5) illustrate SISC-SSI results in different detection SNR and the reconstructed results with different speckle’s numbers are shown in Figs. 6(c1)6(c5), which are similar to the results shown in Figs. 3(g1)3(g5) and 4(a)4(f). Therefore, we further demonstrate the feasibility of the SISC-SSI approach for practical applications.

Fig. 6. (color online) Numerical experimental demonstration of SISC-SSI for a gray-scale object. (a) Original object. (b1)–(b5) The results of SISC-SSI in the detection SNR = ∞, 20 dB, 15 dB, 10 dB, and 5 dB, respectively, and the speckle’s number is 5. (c1)–(c5) The results of SISC-SSI when the speckle’s number is 1, 5, 10, 15, and 30, respectively, and the detection SNR is 10 dB.

Generally speaking, similar to super-resolution imaging via sparsity constraint,[35,9] the object’s sparsity is mainly utilized by the method of SISC-SSI. By random sparse speckle illumination, a complex target is divided into many sparse objects so that the sparse reconstruction algorithm is valid to super-resolution reconstruction. Compared with point scanning imaging (the result shown in Figs. 4(a) and 6(c1)), like spinning disk confocal and multi-focal structured illumination microscopy,[14,22] although the imaging quality is degraded slightly as the speckle’s number is properly increased (Figs. 4(b)4(f) and 6(c2)6(c5)), the measurement number N can be dramatically decreased. Our reconstruction method has utilized the object’s sparsity and the imaging resolution depends on the sparse degree of objects (namely, the speckle number per frame), which is different from point scanning imaging. Searching for better reconstruction algorithm is the main direction of further improvement of our method. What is more, some ideas of SISC-SSI may be adopted by ghost imaging to further improve the imaging resolution, such as sparse speckle illumination and multiple-input detection, which will be our next work.

4. Conclusion

We demonstrate the reconstruction of images borne on sparse speckle illumination and sparsity constraint at a resolution greatly exceeding the finest resolution defined by the optical system’s diffraction limit. We also show that the method of super-resolution imaging is robust to noise and the coded pattern’s sparsity has an obvious influence on the reconstruction quality. This technique will be useful to microscopy in biology, material, and medical sciences.

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